PRACTICAL REPORT SAMPLE

A practical report is a document that describes the process and results of a scientific experiment or research project. It typically includes an introduction, a methods section, a results section, and a discussion of the findings. A practical report sample might look something like this:

### Introduction

The goal of this experiment was to determine the effect of temperature on the reaction rate of enzymes. Enzymes are proteins that catalyze chemical reactions in living organisms, and their activity is affected by a variety of factors including temperature. In this experiment, we tested the effect of temperature on the activity of the enzyme catalase.

### Methods

We began by obtaining a sample of liver tissue, which contains high levels of the enzyme catalase. We then extracted the enzyme by grinding the liver tissue in a mortar and pestle and suspending it in a buffer solution.

Next, we set up a series of test tubes containing the enzyme solution and a substrate (hydrogen peroxide) that would be broken down by the enzyme. We then incubated the test tubes at different temperatures (4°C, 25°C, 37°C, and 50°C) for 15 minutes.

After incubation, we measured the rate of the reaction by measuring the amount of oxygen produced over a period of time. We used a gas sensor to measure the concentration of oxygen in the headspace of each test tube and recorded the data.

### Results

Our results showed that the reaction rate of the enzyme increased as the temperature increased. The highest reaction rate was observed at 50°C, followed by 37°C, 25°C, and finally 4°C. The data is shown in the graph below.

[Insert graph here]

### Discussion

Our results support the hypothesis that temperature has a positive effect on the reaction rate of enzymes. This is consistent with the theory that enzymes have an optimal temperature at which they function most efficiently. In the case of catalase, this appears to be around 50°C.

However, it is important to note that enzymes can be denatured at high temperatures, which can cause them to lose their shape and function. In our experiment, it is possible that the enzyme became denatured at the highest temperature, leading to a decrease in reaction rate.

In conclusion, our experiment demonstrated the effect of temperature on the activity of the enzyme catalase. Further research is needed to determine the optimal temperature range for this enzyme and to understand the mechanisms underlying its temperature sensitivity.